HLA Typing

High Resolution HLA typing

HLA comprehensive high resolution typing (NGS typing of HLA-A,B,C,DRB1,DRB3/4/5, DQA1, DQB1, DPA1, and DPB1 loci)
HLA-A high resolution
HLA-B high resolution
HLA-C high resolution
HLA-DRB1 high resolution
HLA-DRB3/4/5 high resolution
HLA-DQA1 high resolution
HLA-DQB1 high resolution
HLA-DPA1 high resolution
HLA-DPB1 high resolution

To determine HLA matching between patient and donor for hematopoietic stem cell transplantation.
To determine sequence-level HLA matching between patient and donor for solid organ transplantation.
To identify specific HLA types required for vaccine and immunotherapy.
To diagnose certain HLA-associated diseases and drug-induced hypersensitivity.

Next-generation sequencing (NGS) is used to determine the whole HLA gene sequences including all exons and introns.
Comprehensive NGS typing includes all eleven major histocompatibility HLA genes: HLA-A, B, C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1.
Every sample is concurrently typed by rSSO for all 11 HLA loci to corroborate NGS HLA types and to confirm homozygosity.
HLA typing results are reported up to three-field format (e.g., HLA-A*01:01:01).
Comprehensive patient and donor typing report identifies the matched donor allele at each locus as well as total loci.
Typing report identifies mismatches at two field levels per locus with Graft-versus-Host (GvH) or Host-versus-Graft (Rejection) directions.
Typing report includes T cell epitope (TCE) mismatches at HLA-DPB1 locus.
Serological equivalents are provided in the NGS typing report to facilitate the interpretation of HLA allotype and donor-specific HLA antibodies.
Expert consultation is available for all donor searches and selection.

(assuming samples received before 10:00 am)

HLA class I (HLA-A, B, C) intermediate resolution typing
HLA class II (HLA-DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1) intermediate resolution typing

To determine HLA types of solid organ transplant recipient and donor candidates.
To evaluate living and deceased donors for solid organ transplants.
To screen unrelated donors for hematopoietic stem cell transplantation.
To screen for certain HLA-associated diseases.

Minimal ambiguity is achieved by using Luminex-based high definition rSSO bead assay to type all 11 HLA loci.
A high throughput liquid handling system and a 96-well reaction plate is used to minimize inter-assay variations.
The intermediate resolution typing report lists:

1. NMDP ambiguity codes and their corresponding translations.
2. DNA typing and serological equivalents to facilitate the interpretation HLA allotypes and donor-specific HLA antibodies.
3. The probability of finding a zero HLA-ABDR mismatched donor offer from UNOS within a year.

Comprehensive patient and donor typing report identifies the mismatched donor antigen at each locus as well as for total loci.
Deceased donors are concurrently typed by rSSO and 384-well formatted real-time PCR methods for all 11 HLA loci to produce accurate and timely results.

(assuming samples received before 10:00 am)